Difference between revisions of "Part:BBa K4060021:Design"

 
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
a
+
We replace the native signal peptide sequence of aprN with pelB leader sequence by PCR, restriction enzyme digestion and ligation.
 +
With pelB leader sequence, it can increase the secretion of Nattokinase.
  
  
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===Source===
 
===Source===
  
−
a
+
Please refer to iGEM part: BBa_J32015 and BBa_K4060020
  
 
===References===
 
===References===

Latest revision as of 08:16, 4 December 2021


pelB leader sequence with aprN (without native signal peptide sequence)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 67
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 67
    Illegal NotI site found at 211
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 67
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 67
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 345
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 349


Design Notes

We replace the native signal peptide sequence of aprN with pelB leader sequence by PCR, restriction enzyme digestion and ligation. With pelB leader sequence, it can increase the secretion of Nattokinase.


Source

Please refer to iGEM part: BBa_J32015 and BBa_K4060020

References