Difference between revisions of "Part:BBa K3726000"
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This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase. | This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase. | ||
− | The codified enzyme (NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2. | + | The codified enzyme from <i>Streptomyces sp. CL190</i>.(NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2. |
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− | <span class='h3bb'>Sequence | + | <span class='h3bb'><b>Sequence & Features</b></span> |
<partinfo>BBa_K3726000 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3726000 SequenceAndFeatures</partinfo> | ||
Latest revision as of 09:58, 2 October 2021
CDS_NphT7
This part corresponds with the protein coding sequence for an Acetoacetyl CoA synthase.
The codified enzyme from Streptomyces sp. CL190.(NphT7) catalyzes the irreversible condensation-decarboxylation step of 1 molecule of acetyl-coA with Malonyl-coA to transform them into Acetoacetyl-coA and CO2.
Sequence & Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 461
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 461
Illegal PstI site found at 366
Illegal PstI site found at 614
Illegal NgoMIV site found at 105
Illegal NgoMIV site found at 205
Illegal NgoMIV site found at 511
Illegal AgeI site found at 883 - 1000COMPATIBLE WITH RFC[1000]