Difference between revisions of "Part:BBa K3880009"
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− | + | Sep39 is a major protease involved in SCUT-3’s keratin hydrolysis, and previous work showed that its overexpression could increase SCUT-Osep39’s keratinase activity. To further improve the degradation efficiency without influencing the growth rate, we attempt to balance the expression quantity of CDO1 and Sep39 in SCUT-3 through replacing the constitutive promoter with well-characterized promoters. | |
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Latest revision as of 01:18, 18 October 2021
Sep39(protease)
Sep39 is a major protease involved in SCUT-3’s keratin hydrolysis, and previous work showed that its overexpression could increase SCUT-Osep39’s keratinase activity. To further improve the degradation efficiency without influencing the growth rate, we attempt to balance the expression quantity of CDO1 and Sep39 in SCUT-3 through replacing the constitutive promoter with well-characterized promoters.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 482
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 61
Illegal NgoMIV site found at 121
Illegal NgoMIV site found at 661
Illegal NgoMIV site found at 796 - 1000COMPATIBLE WITH RFC[1000]