Difference between revisions of "Part:BBa K4032005"

 
 
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<partinfo>BBa_K4032005 short</partinfo>
 
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This part contains the gene of the α-galactosidase-RFP fusion protein.
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===Usage and Biology===
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This enzyme catalyzes the hydrolysis of terminal, non-reducing α-D-galactose residues in α-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. For more information, see [https://www.uniprot.org/uniprot/P06720 UniProt EC:3.2.1.22].
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Red fluorescence of RFP is observed not only with a fluorescence microscope but also under the natural light source.
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The lac promoter, the RBS and the double terminator are added to this part, forming <partinfo>BBa_K4032101</partinfo>.
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===Design===
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This part expresses the α-galactosidase-RFP fusion protein.
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α-galactosidase is from ''E. coli'' and RFP is from <partinfo>BBa_J04450</partinfo>.
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We removed the stop codon of the α-galactosidase gene and the start codon of the RFP gene and connected the two ends.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 13:23, 21 October 2021


α-galactosidase-RFP

This part contains the gene of the α-galactosidase-RFP fusion protein.

Usage and Biology

This enzyme catalyzes the hydrolysis of terminal, non-reducing α-D-galactose residues in α-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. For more information, see UniProt EC:3.2.1.22.

Red fluorescence of RFP is observed not only with a fluorescence microscope but also under the natural light source.

The lac promoter, the RBS and the double terminator are added to this part, forming BBa_K4032101.

Design

This part expresses the α-galactosidase-RFP fusion protein.

α-galactosidase is from E. coli and RFP is from BBa_J04450.

We removed the stop codon of the α-galactosidase gene and the start codon of the RFP gene and connected the two ends.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1905
    Illegal AgeI site found at 2017
  • 1000
    COMPATIBLE WITH RFC[1000]