Difference between revisions of "Part:BBa K3745035"
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− | + | This part encodes LacI promoter and LacI, which could express repressor protein to bind with lac operator and repress transcription. However, when lactose or IPTG is present, the repressor will first combine with lactose or IPTG, and could no longer restrict the transcription of genes downstream of the operon. | |
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+ | In our project, it is used to control the expression on rhlA,B,C, and protein scaffold. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Latest revision as of 10:41, 14 October 2021
LacI promoter and LacI
This part encodes LacI promoter and LacI, which could express repressor protein to bind with lac operator and repress transcription. However, when lactose or IPTG is present, the repressor will first combine with lactose or IPTG, and could no longer restrict the transcription of genes downstream of the operon.
In our project, it is used to control the expression on rhlA,B,C, and protein scaffold.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]