Difference between revisions of "Part:BBa K3804012"

 
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<partinfo>BBa_K3804012 short</partinfo>
 
<partinfo>BBa_K3804012 short</partinfo>
  
This part aims to secrete HBD3(human β-defensin-2) with yeast. In the part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). Especially, High methionine concentrations repress this promoter and transcription occurs below 0.05 mM methionine. By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when MET17 promoter is activated.  
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This part aims to secrete HBD3(human β-defensin-2) with yeast. In this part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). Especially, high methionine concentrations repress this promoter and transcription occurs below 0.05 mM methionine. By connecting the secretion signal peptide to HBD3, this part makes yeast secrete HBD3 when MET17 promoter is activated.  
  
 
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Latest revision as of 06:35, 3 October 2021


HBD3 in yeast regulated by MET17p

This part aims to secrete HBD3(human β-defensin-2) with yeast. In this part, MET17 promoter displays repression of transcriptional expression when methionine or S–adenosylmethionine exist(Møller et al. 2017). Especially, high methionine concentrations repress this promoter and transcription occurs below 0.05 mM methionine. By connecting the secretion signal peptide to HBD3, this part makes yeast secrete HBD3 when MET17 promoter is activated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]