Difference between revisions of "Part:BBa K3804013"
 
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This part aims to secrete HBD3(human β-defensin-2) with yeast. By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when CYC promoter is activated. In the part, CYC promoter is used so that HBD3 is secreted constitutively.  
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This part aims to secrete HBD3(human β-defensin-3) with <i>Saccharomyces cerevisiae</i>. . By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when CYC promoter is activated. In this part, CYC promoter is used so that yeast secrete HBD3 constitutively.  
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===Characterization===
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As shown in Figure 2, HBD3 dramatically affected yeast growth during the exponential phase compared to control. This property was observed particularly at 250 minutes after the inoculation. However, since the final concentration was almost the same, HBD3 secretion was considered to have little effect on the number of <i>S. cerevisiae</i> in the end. Thus, HBD3 secretion yeast is expected to be sufficient for practical implementation.
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[[File:T--UTokyo--HBD3_Yeast.png|550px|thumb|center|Figure2. Effect of HBD3 expression on growth of <i>S. cerevisiae</i>. Orange:HBD3 expressing strain. Blue:control strain.]]<br>
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===Reference===
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[1] J. Harder, J. Bartels, E. Christophers, and J.-M. Schröder, “Isolation and Characterization of Human μ-Defensin-3, a Novel Human Inducible Peptide Antibiotic,” J. Biol. Chem., vol. 276, no. 8, pp. 5707–5713, Feb. 2001, doi: 10.1074/jbc.M008557200.<br>
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[2]T. S. B. Møller et al., “Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter,” Microb. Cell Factories, vol. 16, no. 1, p. 11, Dec. 2017, doi: 10.1186/s12934-017-0627-7.
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Latest revision as of 11:20, 17 October 2021


HBD3_yeast _constitutive expression

This part aims to secrete HBD3(human β-defensin-3) with Saccharomyces cerevisiae. . By connecting the secretion signal peptide to HBD3, yeast secrete HBD3 when CYC promoter is activated. In this part, CYC promoter is used so that yeast secrete HBD3 constitutively.

Characterization

As shown in Figure 2, HBD3 dramatically affected yeast growth during the exponential phase compared to control. This property was observed particularly at 250 minutes after the inoculation. However, since the final concentration was almost the same, HBD3 secretion was considered to have little effect on the number of S. cerevisiae in the end. Thus, HBD3 secretion yeast is expected to be sufficient for practical implementation.

Figure2. Effect of HBD3 expression on growth of S. cerevisiae. Orange:HBD3 expressing strain. Blue:control strain.

Reference

[1] J. Harder, J. Bartels, E. Christophers, and J.-M. Schröder, “Isolation and Characterization of Human μ-Defensin-3, a Novel Human Inducible Peptide Antibiotic,” J. Biol. Chem., vol. 276, no. 8, pp. 5707–5713, Feb. 2001, doi: 10.1074/jbc.M008557200.
[2]T. S. B. Møller et al., “Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter,” Microb. Cell Factories, vol. 16, no. 1, p. 11, Dec. 2017, doi: 10.1186/s12934-017-0627-7.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]