Difference between revisions of "Part:BBa K3804012:Design"
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===Source=== | ===Source=== | ||
− | This part consists of MET17 promoter, kozack, HBD3 and ADH1terminator. | + | This part consists of MET17 promoter, kozack, MFα1tag, HBD3 and ADH1terminator. |
===References=== | ===References=== | ||
+ | TS. Moller, J. Hay, MJ. Saxton, K. Bunting, EI. Petersen, S. Kjaerulff, et al., Human beta-defensin-2 production from S. cerevisiae using the repressible MET17 promoter, Microbial cell factories 16 (1) (2017) 11, https://doi.org/10.1186/s12934-017-0627-7. |
Latest revision as of 12:56, 30 September 2021
HBD3 in yeast regulated by MET17p
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We added stop codon(taa) at the end of HBD3(BBa_K3351002). Thus, the length of the sequence is 848.
Source
This part consists of MET17 promoter, kozack, MFα1tag, HBD3 and ADH1terminator.
References
TS. Moller, J. Hay, MJ. Saxton, K. Bunting, EI. Petersen, S. Kjaerulff, et al., Human beta-defensin-2 production from S. cerevisiae using the repressible MET17 promoter, Microbial cell factories 16 (1) (2017) 11, https://doi.org/10.1186/s12934-017-0627-7.