Difference between revisions of "Part:BBa K3745000"

 
 
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<partinfo>BBa_K3745000 short</partinfo>
 
<partinfo>BBa_K3745000 short</partinfo>
  
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This can be used as a sensor for selecting mutants
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This part is a rhamnose-induced genetic circuit that contains a Rhamnose promoter, an RBS, an ORF of AmpR, and a terminator. It is improved from [https://parts.igem.org/Part:BBa_K1493501 BBa_K1493501]by using Gibson assembly to replace the GFP with AmpR.
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===Usage and Biology===
 
===Usage and Biology===
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In our project, it is a vital part of the biosensor. After rhamnolipid is hydrolysis to rhamnose, rhamnose could induce this genetic circuit to express the ampicillin resistance gene. This mechanism increases the evolutionary fitness of the strains that have a high rhamnolipid yield (which means more rhamnose could be hydrolysis and induce the expression of AmpR) in culture with ampicillin and enables the strains with favorable mutations to be automatically selected.
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===Qualititive Characterization===
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The following graph demonstrates the validity of this part. With higher rhamnose concentration, the concentration of the bacteria fluid tends to be greater, indicated by a high absorbance at OD600. The absorbance of culture with 0/20/40/60/80ul rhamnose added is measured every hour after liquid inoculation.
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"https://2021.igem.org/wiki/images/a/ae/T--BNDS_China--Results_BBa_K3745000.png"
  
 
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Latest revision as of 01:14, 22 October 2021


Prha-Amp

This part is a rhamnose-induced genetic circuit that contains a Rhamnose promoter, an RBS, an ORF of AmpR, and a terminator. It is improved from BBa_K1493501by using Gibson assembly to replace the GFP with AmpR.


Usage and Biology

In our project, it is a vital part of the biosensor. After rhamnolipid is hydrolysis to rhamnose, rhamnose could induce this genetic circuit to express the ampicillin resistance gene. This mechanism increases the evolutionary fitness of the strains that have a high rhamnolipid yield (which means more rhamnose could be hydrolysis and induce the expression of AmpR) in culture with ampicillin and enables the strains with favorable mutations to be automatically selected.

Qualititive Characterization

The following graph demonstrates the validity of this part. With higher rhamnose concentration, the concentration of the bacteria fluid tends to be greater, indicated by a high absorbance at OD600. The absorbance of culture with 0/20/40/60/80ul rhamnose added is measured every hour after liquid inoculation.

"T--BNDS_China--Results_BBa_K3745000.png"

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 869