Difference between revisions of "Part:BBa K3804010:Design"
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===Source=== | ===Source=== | ||
− | BBa_K1024002, LasR(<bbpart>BBa_C0179</bbpart>), pLas(<bbpart>BBa_K3804004/bbpart>), ADH1 terminator(<bbpart>BBa_J63002</bbpart>) and cyc1terminator(<bbpart>BBa_K1462070</bbpart>). | + | Reporter for quorum sensing systems(<bbpart>BBa_K1024002</bbpart>), LasR(<bbpart>BBa_C0179</bbpart>), pLas(<bbpart>BBa_K3804004</bbpart>), ADH1 terminator(<bbpart>BBa_J63002</bbpart>) and cyc1terminator(<bbpart>BBa_K1462070</bbpart>). |
===References=== | ===References=== |
Latest revision as of 09:00, 29 September 2021
Reporter for quorum sensing systems in yeast with LasR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2567
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1580
Illegal BamHI site found at 2738 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1928
Illegal AgeI site found at 2125 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 506
Illegal BsaI site found at 893
Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 547
Illegal BsaI.rc site found at 934
Illegal BsaI.rc site found at 1321
Design Notes
This part is based on BBa_K1024002. We deleted LuxR and pLux and added LasR, ADH1 terminator, pLas and cyc1 terminator.
Source
Reporter for quorum sensing systems(BBa_K1024002), LasR(BBa_C0179), pLas(BBa_K3804004), ADH1 terminator(BBa_J63002) and cyc1terminator(BBa_K1462070).