Difference between revisions of "Part:BBa K3722004"

 
 
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<partinfo>BBa_K3722004 short</partinfo>
 
<partinfo>BBa_K3722004 short</partinfo>
  
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A linker sequence belongs to our fusion protein Fre-Linker-MaFMO, coding a rigid linker between Fre and MaFMO.
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A linker sequence belongs to our fusion protein Fre-Linker-SttH, coding a rigid linker between Fre and SttH.
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===Biology===
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It is a rigid peptide, which is used to completely isolate the two connected proteins and maintain their independent functions, because some flexible peptides will cause the expression of the fusion protein to decrease or the activity to be lost. It should be noted that rigid peptides cannot be used for two interacting proteins.
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<html>
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    <figure>
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        <img src="https://2021.igem.org/wiki/images/e/ee/T--NWU-CHINA-A--part04.png" width="80%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">
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        </p>
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        </figcaption>
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    </figure>
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</html>
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===Usage===
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The part was used to construct SttH and Fre fusion enzyme to enhance the solubility and activity of SttH. Due to the limited time, we did not characterize this part. As a result, there is a lack of data about this part.
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<html>
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    <figure>
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        <img src="https://2021.igem.org/wiki/images/2/2b/T--NWU-CHINA-A--part01.png" width="100%" style="float:center">
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        <figcaption>
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        <p style="font-size:1rem">
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        </p>
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        </figcaption>
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    </figure>
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</html>
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3722004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3722004 SequenceAndFeatures</partinfo>
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Latest revision as of 21:17, 21 October 2021


A linker sequence in fusion protein Fre-Linker-SttH

A linker sequence belongs to our fusion protein Fre-Linker-SttH, coding a rigid linker between Fre and SttH.


Biology

It is a rigid peptide, which is used to completely isolate the two connected proteins and maintain their independent functions, because some flexible peptides will cause the expression of the fusion protein to decrease or the activity to be lost. It should be noted that rigid peptides cannot be used for two interacting proteins.


Usage

The part was used to construct SttH and Fre fusion enzyme to enhance the solubility and activity of SttH. Due to the limited time, we did not characterize this part. As a result, there is a lack of data about this part.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]