Difference between revisions of "Part:BBa K3725010:Experience"

 
 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<strong>Experience</strong>
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how you used this part and how it worked out.
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The T7 Phytophthora Trigger (BBa_K3725040) and the Phytophthora Toehold w/ GFP Reporter are intended to be compatible with each other and used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was greater and had a statistically significant difference in comparison to cells transformed with only the toehold part and pUC19 plasmid.
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<center>[[File:T--Lambert GA--PhytoIPTGFluorescenceDataGraph.jpeg|500px]]</center>
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<center><I>Figure 2. Mean fluorescence/OD of IPTG-induced Phytophthora dual plasmid transformation compared to toehold and pUC19 with SEM error bars. DP stands for dual plasmid, TH stands for toehold only. Ran at gain of 60.</I></center>
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===Applications of BBa_K3725010===
 
===Applications of BBa_K3725010===

Latest revision as of 22:35, 21 October 2021


Experience
The T7 Phytophthora Trigger (BBa_K3725040) and the Phytophthora Toehold w/ GFP Reporter are intended to be compatible with each other and used in conjunction. To test the compatibility of the trigger sequence with the toehold sequence, a dual-plasmid transformation was performed, and fluorescence was measured in comparison with cells transformed with only the toehold part and pUC19 (positive control). All samples were divided by optical density to obtain a standardized unit. The fluorescence per optical density unit measurement of cells transformed with both the toehold and trigger sequences was greater and had a statistically significant difference in comparison to cells transformed with only the toehold part and pUC19 plasmid.

T--Lambert GA--PhytoIPTGFluorescenceDataGraph.jpeg


Figure 2. Mean fluorescence/OD of IPTG-induced Phytophthora dual plasmid transformation compared to toehold and pUC19 with SEM error bars. DP stands for dual plasmid, TH stands for toehold only. Ran at gain of 60.



Applications of BBa_K3725010

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