Difference between revisions of "Part:BBa K3788002"

 
 
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<partinfo>BBa_K3788002 short</partinfo>
 
<partinfo>BBa_K3788002 short</partinfo>
  
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BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis.
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This chaperon have a histidin tag in Nter to allow to purified the protein P20 and allow the detection and specific identification of P20 by WesterBlot. The gene p20 was optimised for E. coli K12.
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<p> BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis.
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This chaperon has a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12. </p>
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<p></p>
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<p><u> Expected size </u>:</p>
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<p> 6his_<i>p20</i> gene is 570bp </p>
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<p> 6His_P20 protein is 20 kDa </p>
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<u>P20 chaperon:</u>
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<p> Was discovered in <i>B.thuringiensis</i>, a p20 gene in operon with <i>cry11Aa</i> and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). <b>The co-production of P20 and Cyt1Aa in <i>E.coli</i> would limit its toxic effects in the producer cell.</b>  </p>
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Latest revision as of 09:06, 21 October 2021


6His-P20


BBa_K3788002 sequence is coding for P20 a chaperone protein in Bacillus thuringiensis. This chaperon has a 6x Histine tag in N-ter to allow purification of the protein P20and specific detection by Western blot. The gene p20 was optimised for E. coli K12.

Expected size :

6his_p20 gene is 570bp

6His_P20 protein is 20 kDa


P20 chaperon:

Was discovered in B.thuringiensis, a p20 gene in operon with cry11Aa and <i<cyt1Aa</i>. Thus, the P20 protein was shown to be necessary for the formation of inclusion bodies of Cry11Aa and Cyt1Aa. P20 would allow a greater production of these toxins and allow their stability (Cyt1Aa would be protected from the proteolytic action before its maturation). The co-production of P20 and Cyt1Aa in E.coli would limit its toxic effects in the producer cell.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 154
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]