Difference between revisions of "Part:BBa K4073002"

 
 
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<partinfo>BBa_K4073002 short</partinfo>
  
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This composite part consists of a CrGDPH3 salt-inducible promoter, a strong RBS, the AtHKT1 gene, and a terminator. The regulation of the expression of the AtHKT1 gene is the function of this part. The CrGPDH3 salt-inducible promoter was used for the overexpression of proteins in a transgene in microalga Chlamydia reinhardtii.  We then decided to utilize this promoter because of its high NaCl response elements. The RBS initiates translation of the HKT1 gene, which is essential for its protein synthesis. Finally, we used a composite terminator which signals the end of transcription in our part. This is essential, as if we did not utilize a terminator, the AtHKT1 gene could potentially be over-expressed, leading to decreased salt concentrations which could inhibit plant growth (salt is an essential nutrient used in plant growth).
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4073002 SequenceAndFeatures</partinfo>
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https://static.igem.org/mediawiki/parts/0/0c/T--WVHS_SanDiego_CA--mediumRBSnew.png
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===Functional Parameters===
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<partinfo>BBa_K4073002 parameters</partinfo>
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Latest revision as of 20:24, 21 October 2021

BBa_K4073002 regulates salt tolerance in plants.

This composite part consists of a CrGDPH3 salt-inducible promoter, a strong RBS, the AtHKT1 gene, and a terminator. The regulation of the expression of the AtHKT1 gene is the function of this part. The CrGPDH3 salt-inducible promoter was used for the overexpression of proteins in a transgene in microalga Chlamydia reinhardtii. We then decided to utilize this promoter because of its high NaCl response elements. The RBS initiates translation of the HKT1 gene, which is essential for its protein synthesis. Finally, we used a composite terminator which signals the end of transcription in our part. This is essential, as if we did not utilize a terminator, the AtHKT1 gene could potentially be over-expressed, leading to decreased salt concentrations which could inhibit plant growth (salt is an essential nutrient used in plant growth).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 755
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1913
    Illegal BsaI.rc site found at 225

T--WVHS_SanDiego_CA--mediumRBSnew.png