Difference between revisions of "Part:BBa K3718009"

 
(An example product of our toolkit part II after cloning)
 
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<partinfo>BBa_K3718009 short</partinfo>
 
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An example product of our toolkit part II after cloning
 
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After the experiments of the Modular Receptor Platform (MRP) expression and rescue KO <I>E. coli</I> have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, arcA and iclR, which will result in an increase in growth rate and a visible green color under UV.
 
  
  
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<p>After the experiments of the Modular Receptor Platform (MRP) expression and rescue KO <I>E. coli</I> have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, arcA and iclR, which will result in an increase in growth rate and a visible green color under UV.</p>

Latest revision as of 10:56, 26 September 2021


Demonstrative circuit of tool kit part 2



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


After the experiments of the Modular Receptor Platform (MRP) expression and rescue KO E. coli have proven to be successful, we will use caffeine-sensitive MRP as a testing part to model the system. Caffeine will be used to homodimerize the receptors, and is expected to induce the expression of downstream genes. This model will be used to conduct three experiments to confirm the functionality of the tool. By cloning in an antibody VHH gene against caffeine and gfp as a desired gene to be expressed, we expect the platform is activated in the presence of caffeine to express gfp, arcA and iclR, which will result in an increase in growth rate and a visible green color under UV.