Difference between revisions of "Part:BBa K3766034:Design"

 
 
Line 4: Line 4:
  
 
<partinfo>BBa_K3766034 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3766034 SequenceAndFeatures</partinfo>
 +
  
  
 
===Design Notes===
 
===Design Notes===
−
Salis
+
This sequence has been specifically designed using the [https://www.denovodna.com/ Salis Lab / De Novo DNA RBS Library Calculator v2.1.1] [1, 2, 3].
−
 
+
−
 
+
  
 
===Source===
 
===Source===
Line 16: Line 15:
  
 
===References===
 
===References===
 +
[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.
 +
 +
[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.
 +
 +
[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.

Latest revision as of 09:43, 20 October 2021


Synthetic RBS designed for sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This sequence has been specifically designed using the Salis Lab / De Novo DNA RBS Library Calculator v2.1.1 [1, 2, 3].

Source

DNA synthesis

References

[1] Reis AC, Salis HM. An automated model test system for systematic development and improvement of gene expression models. ACS synthetic biology (2020) 9: 3145–3156.

[2] Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM. Efficient search, mapping, and optimization of multi-protein genetic systems in diverse bacteria. Molecular Systems Biology (2014) 10: 731.

[3] Ng CY, Farasat I, Maranas CD, Salis HM. Rational design of a synthetic Entner-Doudoroff pathway for improved and controllable NADPH regeneration. Metabolic Engineering (2015) 29: 86–96.