Difference between revisions of "Part:BBa K3766022:Design"

 
 
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===Design Notes===
 
===Design Notes===
−
to do
+
T7 CGG promoter (TAATACCGGTCACTATA) [1] + GG at 3' end [2]
−
 
+
−
 
+
  
 
===Source===
 
===Source===
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===References===
 
===References===
 +
[1] Meyer AJ, Ellefson JW, Ellington AD. Directed evolution of a panel of orthogonal T7 RNA Polymerase variants for ''in vivo'' or ''in vitro'' synthetic circuitry. ACS synthetic biology (2015) 4: 1070–1076.
 +
 +
[2] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.

Latest revision as of 10:13, 20 October 2021


T7 CGG promoter (reverse orientation)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

T7 CGG promoter (TAATACCGGTCACTATA) [1] + GG at 3' end [2]

Source

DNA synthesis

References

[1] Meyer AJ, Ellefson JW, Ellington AD. Directed evolution of a panel of orthogonal T7 RNA Polymerase variants for in vivo or in vitro synthetic circuitry. ACS synthetic biology (2015) 4: 1070–1076.

[2] Imburgio D, Rong M, Ma K, McAllister WT. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry (2000) 39, 10419-10430.