Difference between revisions of "Part:BBa K3866019"
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<partinfo>BBa_K3866019 short</partinfo> | <partinfo>BBa_K3866019 short</partinfo> | ||
− | [[File:T--Thessaly-- | + | [[File:T--Thessaly--stuffer.a2.png|700px|thumb|none|<i><b>Fig.2:</b>An alpha2 vector with the pdgb stuffer a2.</i>]] |
===Usage and Biology=== | ===Usage and Biology=== | ||
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===References=== | ===References=== | ||
− | + | *Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, et al. (2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLOS ONE 6(7): e21622. https://doi.org/10.1371/journal.pone.0021622 |
Latest revision as of 11:02, 29 September 2021
Stuffer PDGB A2
Usage and Biology
This translational unit is consisted of and alpha 2 vector with a stuffer. Stuffer is a part of DNA that is coding for nothing. It is used in order to swap inserts from alpha 2 or alpha2R vectors into omega vectors. This serves the GB cloning procedure
Design Notes
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in alpha 2 vector BBa_K3505009 and has overhangs compatible for GoldenBraid cloning.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Source
Synthesized by Twist Biosciences.
References
- Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, et al. (2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLOS ONE 6(7): e21622. https://doi.org/10.1371/journal.pone.0021622