Difference between revisions of "Part:BBa K3866020"
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<partinfo>BBa_K3866020 short</partinfo>
 
<partinfo>BBa_K3866020 short</partinfo>
  
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
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[[File:T--Thessaly--omega2.CymR.Stuffer.png|700px|thumb|none|<i><b>Fig.2:</b>The omega 2 vector with two translational units. The one is J23115-CymR-double terminator and the other one is the stuffer.</i>]]
  
 
===Usage and Biology===
 
===Usage and Biology===
  
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This composite part is consisted of the translational unit of the strong constitutive promoter J23115 <bbpart>BBa_K3505012</bbpart> and the Cym Repressor <bbpart>BBa K415202</bbpart> and the transcriptional unit with the stuffer <bbpart>BBa_K3866019</bbpart>, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector <bbpart>BBa_K3505011</bbpart>.
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<p>This composite part is consisted of the translational unit of the strong constitutive promoter J23115 <bbpart>BBa_K3505012</bbpart>and the Cym Repressor <bbpart>BBa_K415202</bbpart>and the transcriptional unit with the stuffer <bbpart>BBa_K3866019</bbpart>, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector <bbpart>BBa_K3505011</bbpart>.
  
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===Design Notes===
 
===Design Notes===
 
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for GoldenBraid cloning.
 
The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector <bbpart>BBa_K3505008</bbpart> and has overhangs compatible for GoldenBraid cloning.
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===References===
 
===References===
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i
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*Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, et al. (2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLOS ONE 6(7): e21622. https://doi.org/10.1371/journal.pone.0021622
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*Choi, Y. J., Morel, L., Le François, T., Bourque, D., Bourget, L., Groleau, D., Massie, B., & Míguez, C. B. (2010). Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Applied and environmental microbiology, 76(15), 5058–5066. https://doi.org/10.1128/AEM.00413-10

Latest revision as of 11:03, 29 September 2021


J23115-CymR-Stuffer a2

Fig.2:The omega 2 vector with two translational units. The one is J23115-CymR-double terminator and the other one is the stuffer.

Usage and Biology

This composite part is consisted of the translational unit of the strong constitutive promoter J23115 BBa_K3505012and the Cym Repressor BBa_K415202and the transcriptional unit with the stuffer BBa_K3866019, which is a part of DNA non-coding with a length close to 150 basepairs. The stuffer is used so that we can swap the insert from the alpha 2 vector into the omega 2 vector BBa_K3505011.

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in omega2 vector BBa_K3505008 and has overhangs compatible for GoldenBraid cloning.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 418
    Illegal XhoI site found at 199
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 101

Source

Synthesized by Twist Biosciences.

References

  • Sarrion-Perdigones A, Falconi EE, Zandalinas SI, Juárez P, Fernández-del-Carmen A, et al. (2011) GoldenBraid: An Iterative Cloning System for Standardized Assembly of Reusable Genetic Modules. PLOS ONE 6(7): e21622. https://doi.org/10.1371/journal.pone.0021622
  • Choi, Y. J., Morel, L., Le François, T., Bourque, D., Bourget, L., Groleau, D., Massie, B., & Míguez, C. B. (2010). Novel, versatile, and tightly regulated expression system for Escherichia coli strains. Applied and environmental microbiology, 76(15), 5058–5066. https://doi.org/10.1128/AEM.00413-10