Difference between revisions of "Part:BBa K3866011"
 
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<p>MazF toxin is used in the toxin-antitoxin system with mazE anti-toxin.This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity
 
<p>MazF toxin is used in the toxin-antitoxin system with mazE anti-toxin.This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity
  
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
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[[File:T--Thessaly--MazF.png|700px|thumb|none|<i><b>Fig.2:</b>MazF toxin into PUPD2 with GB compatible ends.</i>]]
  
 
===Usage and Biology===
 
===Usage and Biology===
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===References===
 
===References===
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*Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107

Latest revision as of 19:08, 26 September 2021


MazF toxin GB compatible with B3-B5

MazF toxin is used in the toxin-antitoxin system with mazE anti-toxin.This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity

Fig.2:MazF toxin into PUPD2 with GB compatible ends.

Usage and Biology

The MazEF system is an extensively studied type II TA module. MazF homologs are well known as ribosome-independent toxins and have sequence-specific endoribonucleases that can cleave mRNA sequences in a ribosome-independent manner.



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.

Fig.1:The overhangs of this part in the GoldenBraid Grammar


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

  • Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107