Difference between revisions of "Part:BBa K3866008"
 
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<p>MazE antitoxin is used in the toxin-antitoxin system with mazF toxin. This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity.
 
<p>MazE antitoxin is used in the toxin-antitoxin system with mazF toxin. This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity.
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[[File:T--Thessaly--AndersonRBS-snap.png|700px|thumb|none|<i><b>Fig.2:</b>J23115 with RBS</i>]]
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[[File:T--Thessaly--MazE.png|700px|thumb|none|<i><b>Fig.2:</b>MazE antitoxin with compatible ends for Golden Braid Cloning</i>]]
  
 
===Usage and Biology===
 
===Usage and Biology===
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===Design Notes===
 
===Design Notes===
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.
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The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 <bbpart>BBa_K3505007</bbpart> and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.
 
[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
 
[[File:T--Thessaly--GB-GGAG-AATG.jpeg|700px|thumb|none|<i><b>Fig.1:</b>The overhangs of this part in the GoldenBraid Grammar</i>]]
  
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===Sequence and Features===
 
===Sequence and Features===
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<partinfo>BBa_K3866003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3866008 SequenceAndFeatures</partinfo>
  
 
===Source===
 
===Source===
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===References===
 
===References===
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*Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107

Latest revision as of 19:20, 26 September 2021


MazE antitoxin GB compatible with B3-B5

MazE antitoxin is used in the toxin-antitoxin system with mazF toxin. This is a toxin-antitoxin system type II, widely used at most labs. It binds to the MazF endoribonuclease toxin and neutralizes its endoribonuclease activity.

Fig.2:MazE antitoxin with compatible ends for Golden Braid Cloning

Usage and Biology

The MazEF system is an extensively studied type II TA module.Toxins in type II TA systems not only inhibit DNA, RNA, or protein synthesis but are also involved in antibiotic resistance, biofilm formation, and persistent cell formation in the plasmid-based systems



Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is cloned in pUPD2 BBa_K3505007 and has overhangs compatible for GoldenBraid cloning. The Promoter has position B3-B5.

Fig.1:The overhangs of this part in the GoldenBraid Grammar


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Source

Synthesized by Twist Biosciences.

References

  • Choi, W., Yamaguchi, Y., Park, J.-Y., Park, S.-H., Lee, H.-W., Lim, B.-K., … Park, J.-H. (2021). Functional Characterization of the mazEF Toxin-Antitoxin System in the Pathogenic Bacterium Agrobacterium tumefaciens. Microorganisms, 9(5), 1107. doi:10.3390/microorganisms9051107