Difference between revisions of "Part:BBa K3853012"
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<partinfo>BBa_K3853012 short</partinfo> | <partinfo>BBa_K3853012 short</partinfo> | ||
| − | + | This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as <partinfo>BBa_I746916</partinfo>. Using the <b>Thermo GeneArt</b> online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in <i>Pichia pastoris</i>. We use <partinfo>BBa_K3853055</partinfo> to construct the expression system to express and purify the protein. | |
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Latest revision as of 10:01, 17 October 2021
sfGFP Codon adapted for Pichia pastoris
This is the coding sequence of superfolder GFP (Pedelacq et al (2006): "Engineering and characterization of a superfolder green fluorescent protein", Nature Biotech 24 (1) January 2006). This part of the amino acid sequence is the same as BBa_I746916. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. We use BBa_K3853055 to construct the expression system to express and purify the protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]