Difference between revisions of "Part:BBa K3792005"

 
 
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{{FSU/ChartsCSS}}
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#modular-promoter-library-chart.column {
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#modular-promoter-library-chart tr:hover th {
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</html>
  
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3792005 short</partinfo>
 
<partinfo>BBa_K3792005 short</partinfo>
  
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The apFAB51 test device does not produce a lot of the mRFP1 protein. The protein, mRFP1, is a red fluorescent protein.  
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<p>
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The apFAB51 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. <strong>The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements</strong>.
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</p>
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<div id="my-chart">
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<table id="modular-promoter-library-chart" class="charts-css column show-labels show-data show-primary-axis show-10-secondary-axes">
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      <caption>Promoter Strength Measurement</caption>
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      <tr>
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        <th scope="row">Negative<br>Control</th>
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        <td style="--size: calc(1000/2000)"><span class="tooltip">Negative Control</span><span class="data">1000</span></td>
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      </tr>
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      <tr>
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      <th scope="row">apFAB40<br>BBa_K3792003</th>
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      <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB40<br>BBa_K3792003</span><span class="data">1700</span></td>
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      </tr>
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      <tr>
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        <th scope="row">apFAB98<br>BBa_K3792004</th>
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        <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB98<br>BBa_K3792004</span><span class="data">1700</span></td>
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      </tr>
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      <tr>
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        <th scope="row">apFAB51<br>BBa_K3792005</th>     
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        <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB51<br>BBa_K3792005</span><span class="data">1700</span></td>
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    </tr>
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  </table>
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  <div class="data-axis">molecules of equivalent PE-Texas Red (MEPTR)</div>
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</div>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3792005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3792005 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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<!-- Uncomment this to enable Functional Parameter display  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K3792005 parameters</partinfo>
 
<partinfo>BBa_K3792005 parameters</partinfo>
 
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Latest revision as of 03:49, 22 October 2021


BIOFAB apFAB51 Measurement Device

The apFAB51 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements.

Promoter Strength Measurement
Negative
Control		 Negative Control1000
apFAB40
BBa_K3792003		 apFAB40
BBa_K37920031700
apFAB98
BBa_K3792004		 apFAB98
BBa_K37920041700
apFAB51
BBa_K3792005		 apFAB51
BBa_K37920051700
molecules of equivalent PE-Texas Red (MEPTR)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 614
    Illegal AgeI site found at 726
  • 1000
    COMPATIBLE WITH RFC[1000]