Difference between revisions of "Part:BBa K3792004"
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+ | {{FSU/ChartsCSS}} | ||
+ | |||
+ | <html> | ||
+ | <style> | ||
+ | #modular-promoter-library-chart.column { | ||
+ | height: 250px; | ||
+ | max-width: 50% !important; | ||
+ | margin: 0 auto; | ||
+ | --labels-size: 2rem; | ||
+ | } | ||
+ | |||
+ | #modular-promoter-library-chart tr { | ||
+ | transition-duration: 0.3s; | ||
+ | } | ||
+ | #modular-promoter-library-chart tr:hover { | ||
+ | background-color: rgba(0, 0, 0, 0.2); | ||
+ | } | ||
+ | #modular-promoter-library-chart tr:hover th { | ||
+ | background-color: rgba(0, 0, 0, 0.4); | ||
+ | color: #fff; | ||
+ | } | ||
+ | |||
+ | #my-chart { | ||
+ | display: grid; | ||
+ | align-items: center; | ||
+ | justify-items: center; | ||
+ | grid-template-columns: 50px 1fr; | ||
+ | grid-template-rows: 250px 50px; | ||
+ | grid-template-areas: | ||
+ | "data-axis chart" | ||
+ | ". primary-axis"; | ||
+ | } | ||
+ | #my-chart > table { | ||
+ | grid-area: chart; | ||
+ | } | ||
+ | #my-chart > .primary-axis { | ||
+ | grid-area: primary-axis; | ||
+ | } | ||
+ | #my-chart > .data-axis { | ||
+ | grid-area: data-axis; | ||
+ | writing-mode: tb-rl; | ||
+ | transform: rotateZ(180deg); | ||
+ | } | ||
+ | </style> | ||
+ | </html> | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K3792004 short</partinfo> | <partinfo>BBa_K3792004 short</partinfo> | ||
− | The apFAB98 | + | <p> |
+ | The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. <strong>The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements</strong>. | ||
+ | </p> | ||
+ | |||
+ | <div id="my-chart"> | ||
+ | <table id="modular-promoter-library-chart" class="charts-css column show-labels show-data show-primary-axis show-10-secondary-axes"> | ||
+ | <caption>Promoter Strength Measurement</caption> | ||
+ | <tr> | ||
+ | <th scope="row">Negative<br>Control</th> | ||
+ | <td style="--size: calc(1000/2000)"><span class="tooltip">Negative Control</span><span class="data">1000</span></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th scope="row">apFAB40<br>BBa_K3792003</th> | ||
+ | <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB40<br>BBa_K3792003</span><span class="data">1700</span></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th scope="row">apFAB98<br>BBa_K3792004</th> | ||
+ | <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB98<br>BBa_K3792004</span><span class="data">1700</span></td> | ||
+ | </tr> | ||
+ | <tr> | ||
+ | <th scope="row">apFAB51<br>BBa_K3792005</th> | ||
+ | <td style="--size: calc(1700/2000)"><span class="tooltip">apFAB51<br>BBa_K3792005</span><span class="data">1700</span></td> | ||
+ | </tr> | ||
+ | </table> | ||
+ | <div class="data-axis">molecules of equivalent PE-Texas Red (MEPTR)</div> | ||
+ | </div> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3792004 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3792004 SequenceAndFeatures</partinfo> | ||
− | + | <!-- Uncomment this to enable Functional Parameter display | |
− | + | ||
− | <!-- Uncomment this to enable Functional Parameter display | + | |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3792004 parameters</partinfo> | <partinfo>BBa_K3792004 parameters</partinfo> | ||
<!-- --> | <!-- --> |
Latest revision as of 03:39, 22 October 2021
BIOFAB apFAB98 Measurement Device
The apFAB98 Measurement Device is composed of a BIOFAB promoter, a ribosome binding site (BBa_B0034), mRFP1 (BBa_E1010), and a terminator (BBa_B0015). The measurement device was inserted into the pSB1K3 backbone using the NEB HiFi DNA Assembly method. The resulting plasmid vectors were used to transform the E. coli NEBExpress chassis. The promoter strength was measured via the amount of fluorescence detected in a flow cytometer. The level of fluorescence will be reported in molecules of equivalent PE-Texas Red (MEPTR) units. The analysis of the flow cytometry data is ongoing and will be shared during the Giant Jamboree. The interactive chart displayed below is a placeholder for the measurements.
Negative Control |
Negative Control1000 |
---|---|
apFAB40 BBa_K3792003 |
apFAB40 BBa_K37920031700 |
apFAB98 BBa_K3792004 |
apFAB98 BBa_K37920041700 |
apFAB51 BBa_K3792005 |
apFAB51 BBa_K37920051700 |
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 615
Illegal AgeI site found at 727 - 1000COMPATIBLE WITH RFC[1000]