Difference between revisions of "Part:BBa K3853000"
 
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<partinfo>BBa_K3853000 short</partinfo>
 
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Manganese Peroxidase(MnP)
 
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Manganese Peroxidase(MnP)is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from <i>Phanerochaete chrysosporium</i>. The gene enables our efficiently Multi-enzyme complex to degrade PE
 
  
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Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn<sup>2+</sup> to Mn<sup>3+</sup>, which would be chelated by ligands like oxalic acid. And the Mn<sup>3+</sup>-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from <i>Phanerochaete chrysosporium</i>. Using the <b>Thermo GeneArt</b> online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in <i>Pichia pastoris</i>. The introduction of this gene helps our system degrade PE effectively.
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===Biology===
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===Usage===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 20:41, 7 October 2021


Manganese Peroxidase (MnP)


Manganese Peroxidase (MnP) is a highly glycosylated lignin peroxidase with heme. It can oxidize Mn2+ to Mn3+, which would be chelated by ligands like oxalic acid. And the Mn3+-ligand chelate compound would be released outside the enzyme for further degrading lignin or other refractory chemicals.This gene comes from Phanerochaete chrysosporium. Using the Thermo GeneArt online tool geneoptimize, we optimize the codon of the protein gene sequence to improve its expression in Pichia pastoris. The introduction of this gene helps our system degrade PE effectively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]