Difference between revisions of "Promoters/Catalog/B. subtilis/Repressible"

(New page: All the promoters on this page are ''B. subtilis'' promoters that are '''negatively regulated''' meaning that increased levels of at least one transcription factor (other than the sigma fa...)
 
 
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All the promoters on this page are ''B. subtilis'' promoters that are '''negatively regulated''' meaning that increased levels of at least one transcription factor (other than the sigma factor) will decrease the activity of these promoters.
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[[Image:NegativePromoter.png|right|200px]]
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The B. subtilis promoters of this section are said negativly regulated promoters, because they can be repressed by the expression of a third party protein. The inhibition can be released by the addition of a molecule, like for the LacI ''E. coli'' promoter.
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In the following biobricks, the proposed promoters are build with the fusion of one or several operons with a &sigma;<sub>A</sub> type contitutive promoter.
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<parttable>promoter_subtilis_negative_sigmaA</parttable>
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<br />
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In the future, we may also find promoters builded with the &sigma;<sub>B</sub> promoter.
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<parttable>promoter_subtilis_negative_sigmaB</parttable>
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<html>
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<style>
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#assembly_plasmid_table {margin-left:auto;margin-right:auto; border: 3px solid #444444;}
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#assembly_plasmid_table td {background-color:#eeeeee; padding: 5px; font-size: 12px;}
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#assembly_plasmid_table th {background-color: #aaaaaa;padding: 5px; font-size:14px;}
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</style>
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</html>

Latest revision as of 04:49, 4 November 2011

NegativePromoter.png

The B. subtilis promoters of this section are said negativly regulated promoters, because they can be repressed by the expression of a third party protein. The inhibition can be released by the addition of a molecule, like for the LacI E. coli promoter.

In the following biobricks, the proposed promoters are build with the fusion of one or several operons with a σA type contitutive promoter.


More...
NameDescriptionPromoter SequencePositive
Regulators
Negative
Regulators
LengthDocStatus
BBa_K090501Gram-Positive IPTG-Inducible Promoter . . . tggaattgtgagcggataacaattaagctt1072054It's complicated
BBa_K143014Promoter Xyl for B.subtilis . . . agtttgtttaaacaacaaactaataggtga822112Not in stock
BBa_K143015Promoter hyper-spank for B. subtilis . . . aatgtgtgtaattgtgagcggataacaatt1012527Not in stock


In the future, we may also find promoters builded with the σB promoter.


There are no parts for this table