Difference between revisions of "Part:BBa K136046"

Latest revision as of 04:46, 8 November 2008

envZ*

EnvZ is a transmembrane histidine kinase that, in response to osmotic changes, phosphorylates OmpR the natural inhibitor of flhDC promotor the master regulator of the flagella synthesis. EnvZ* is here a mutated version which constitutively phosphorylates OmpR. The resulting phenotype of the mutated strain is complete repression of FlhDC expression and then absence of motility for the bacteria.

Image : The described point mutations of EnvZ in motility deficient strains of E. coli (Giraud et al. PLoS Genet. 2008). The mutated EnvZ described here is mutated I281S and present the more severe phenotype.

This part was successfully used in the composite part BBa_K136066

A site EcoRI is present in the part which doesn't comply with the Biobrick standard. We will soon provide a new version of this part without the EcoRI site.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 316
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 316
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 316
Illegal BglII site found at 236
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 316
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 316
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 EnvZ* is here a mutated version which constitutively phosphorylates OmpR. The resulting phenotype of the mutated strain is complete repression of FlhDC expression and then absence of motility for the bacteria.
-[[Image:EnvZmut.png|300px]]
+[[Image:EnvZmut.png|300px]]<br>
 '''Image :''' The described point mutations of EnvZ in motility deficient strains of E. coli (Giraud ''et al.'' PLoS Genet. 2008). The mutated EnvZ described here is mutated I281S and present the more severe phenotype.