Difference between revisions of "Part:BBa K3739032"

Latest revision as of 03:43, 22 October 2021

J23100-B0030-his-CBM-GFP-B0010

CBM can anchor onto cellulose and GFP can show whether CenA anchor or not.

Usage and Biology

CBM

Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from Cellulomonas fimi, which has been successfully expressed in Escherichia coli.

GFP

Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 15
Illegal NheI site found at 38
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 CBM can anchor onto cellulose and GFP can show whether CenA anchor or not.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+=====CBM=====
+Cellulose enzymes have two domains, and the one that helps bind to cellulose is called cellulose binding module (CBM), and therefore it helps our fusion protein bind to cellulose-rich cell wall. Here we choose the CBM of CenA from ''Cellulomonas fimi'', which has been successfully expressed in ''Escherichia coli''.
+=====GFP=====
+Protein tags are peptides genetically fused to the proteins of interest. There are many different kinds of tags developed for different purposes. For labeling experiments, normally the proteins of interest are tagged directly by fluorescent tags. Green fluorescent protein (GFP) make it easier to study protein localization, interactions and dynamics when fusing with other peptides and proteins.
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