Difference between revisions of "Part:BBa K138000"
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| − | <partinfo>BBa_K138000 short</partinfo> | + | <partinfo>BBa_K138000 short</partinfo> <BR> |
| − | + | The NifH promoer is from K.pneumoniae. | |
The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI. | The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI. | ||
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Latest revision as of 10:57, 10 June 2009
NifHp in BioBrick Compatible Leucine Landing Pad
The NifH promoer is from K.pneumoniae.
The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]