Difference between revisions of "Part:BBa K3767004"

Revision as of 18:03, 3 July 2021 (view source) Victor5688 (Talk | contribs) ← Older edit		Latest revision as of 21:19, 18 October 2021 (view source) GriffinWB (Talk | contribs)
(4 intermediate revisions by 2 users not shown)
Line 3:		Line 3:
	<partinfo>BBa_K3767004 short</partinfo>		<partinfo>BBa_K3767004 short</partinfo>
−	Goal/Use: Current Lyme disease testing involves Enzyme-linked immunosorbent assay (ELISA) test which is used to detect Borrelia Burgfedori antibodies after a tick bite. However, by testing for OspA on the tick itself, we hope to be able to identify an earlier diagnosis for the Borrelia Burgfedori bacterium in the case of a tick bite.	+	Current Lyme disease testing involves Enzyme-linked immunosorbent assay (ELISA) test which is used to detect Borrelia Burgfedori antibodies after a tick bite. However, by testing for OspA on the tick itself, we hope to be able to identify an earlier diagnosis for the Borrelia Burgfedori bacterium in the case of a tick bite.
	===Usage and Biology===		===Usage and Biology===
−	Outer surface protein A (OspA) is an abundant lipoprotein that resides on the bacterium Borrelia Burgfedori (Borrelia meaning invasive spiral shaped pathogen), which is carried by ticks and transmitted via a tick bite. Borrelia Burgfedori harbors a plasmid which encodes OspA, which plays a role in the attachment and penetration of the spirochete to the epithelial cells once bitten by the tick(1). The amino acid sequence of OspA begins with residues believed to function as signal sequences for transport, proteolytic processing and lipidation(2). Recent findings have also shown that OspA is important in the inflammation process in Lyme disease, thus possesses both B-cell mitogenic effect and a cytokine-stimulatory effect(1).	+	Outer surface protein A (OspA) is an abundant lipoprotein that resides on the bacterium Borrelia Burgfedori (Borrelia meaning invasive spiral shaped pathogen), which is carried by ticks and transmitted via a tick bite. Borrelia Burgfedori expresses the protein OspA, which plays a role in the attachment and penetration of the spirochete to the epithelial cells once bitten by the tick[[Part:BBa_K3767004#References|<sup>[1]</sup>]]. The amino acid sequence of OspA begins with residues believed to function as signal sequences for transport, proteolytic processing and lipidation[[Part:BBa_K3767004#References|<sup>[2]</sup>]]. [[File:BBa K3767004 OspA colored.png|300px|right|thumb| Figure 1. Colored model of purified OspA from E.Coli.]]Recent findings have also shown that OspA is important in the inflammation process in Lyme disease, thus possesses both B-cell mitogenic effect and a cytokine-stimulatory effect[[Part:BBa_K3767004#References|<sup>[1]</sup>]].
−	OspA is composed with a prolonged fold with 21 anti-parallel beta sheets and a single alpha helix, which are arranged to form the N-terminal, central sheet and C-terminal (3). OspA has a unique folding pattern that includes alternating charge arrays into anti-parallel &#946;-sheet, a potential ligand binding site and a distinctive variable motif (3). OspA normally has a lipidated N-terminal cysteine, a recombinant unlipidated form is soluble in aqueous solution and is still recognized by antibodies from Lyme Disease	+	OspA is composed with a prolonged fold with 21 anti-parallel beta sheets and a single alpha helix, which are arranged to form the N-terminal, central sheet and C-terminal [[Part:BBa_K3767004#References|<sup>[3]</sup>]]. OspA has a unique folding pattern that includes alternating charge arrays into anti-parallel &#946;-sheet, a potential ligand binding site and a distinctive variable motif [[Part:BBa_K3767004#References|<sup>[3]</sup>]]. OspA normally has a lipidated N-terminal cysteine, a recombinant unlipidated form is soluble in aqueous solution and is still recognized by antibodies from Lyme Disease
	<!-- -->		<!-- -->
−	<span class='h3bb'>Sequence and Features</span>	+
	<partinfo>BBa_K3767004 SequenceAndFeatures</partinfo>		<partinfo>BBa_K3767004 SequenceAndFeatures</partinfo>

---

Latest revision as of 21:19, 18 October 2021

Outer Surface Protein A (OspA) from Borrelia Burgdorferi

Current Lyme disease testing involves Enzyme-linked immunosorbent assay (ELISA) test which is used to detect Borrelia Burgfedori antibodies after a tick bite. However, by testing for OspA on the tick itself, we hope to be able to identify an earlier diagnosis for the Borrelia Burgfedori bacterium in the case of a tick bite.

Usage and Biology

Outer surface protein A (OspA) is an abundant lipoprotein that resides on the bacterium Borrelia Burgfedori (Borrelia meaning invasive spiral shaped pathogen), which is carried by ticks and transmitted via a tick bite. Borrelia Burgfedori expresses the protein OspA, which plays a role in the attachment and penetration of the spirochete to the epithelial cells once bitten by the tick^[1]. The amino acid sequence of OspA begins with residues believed to function as signal sequences for transport, proteolytic processing and lipidation^[2]. Recent findings have also shown that OspA is important in the inflammation process in Lyme disease, thus possesses both B-cell mitogenic effect and a cytokine-stimulatory effect^[1].

OspA is composed with a prolonged fold with 21 anti-parallel beta sheets and a single alpha helix, which are arranged to form the N-terminal, central sheet and C-terminal ^[3]. OspA has a unique folding pattern that includes alternating charge arrays into anti-parallel β-sheet, a potential ligand binding site and a distinctive variable motif ^[3]. OspA normally has a lipidated N-terminal cysteine, a recombinant unlipidated form is soluble in aqueous solution and is still recognized by antibodies from Lyme Disease

References

1. Hansson, L., Noppa, L., Nilsson, A., Stromqvist, M., Bergstrom, S. (1994). Expression of Truncated and Full-Length Forms of the Lyme Disease Borrelia Outer Surface Protein A in Escherichia coli, Umed University, Umed, Sweden.

2. Dunn, J., Lade, B., Barbour, A. (1990). Outer Surface Protein A (OspA) from Lyme Disease Spirochete, Borrelia burgdorferi: High Level Expression and Purification of a Soluble Recombinant from of OspA, University of Texas, San Antonio, Texas.

3. Park, J., Prilusky, J., Harel, M and Martz, E. (2015). OspA. Proteopedia [Online].https://proteopedia.org/wiki/index.php/OspA. (Accessed June 19, 2022).

4. Chang, Y-F., Lauderdale, T-L., Lee, W., Shin, J., Jacobson, R., Appel, M and Lein, D. (1993). Expression and secretion of outer surface protein (OSP-A) of Borrelia burgdorferi from Escherichia coli, Cornell University, Ithaca, NY, USA.