Difference between revisions of "Part:BBa K875003"
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This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter. | This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter. | ||
Latest revision as of 07:21, 30 May 2021
CymR
This composite produces constitutively the CymR regulator protein, that binds the Cumate Operator repressing the transcription from the promoter.
1. The cumate gene-switch: a system for regulated expression in mammalian cells Alaka Mullick, Yan Xu, René Warren, Maria Koutroumanis, Claire Guilbault, Sophie Broussau, Félix Malenfant, Lucie Bourget, Linda Lamoureux, Rita Lo, Antoine W Caron, Amelie Pilotte and Bernard Massie ( BMC Biotechnology 2006, 6:43 doi:10.1186/1472-6750-6-43 )
2. Novel, Versatile, and Tightly Regulated Expression System for Escherichia coli Strains Young J. Choi, Lyne Morel, Teffanie Le François Denis Bourque, Lucie Bourget, Denis Groleau, Bernard Massie and Carlos B. Míguez (Applied and Environmental Microbiology, Aug. 2010, p. 5058–5066 Vol.76 doi:10.1128/AEM.00413-10)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 427
Illegal XhoI site found at 208 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 110