Difference between revisions of "Part:BBa K3513006"
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=='''Improved by NFLS 2020'''== | =='''Improved by NFLS 2020'''== | ||
| − | + | ===Biology=== | |
<p>NF-κB is one of the most pathways inside the human body. This part contains five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. NF-κB is the down-stream of TNF signal, so in the experiments we add TNF to induce NF-κB production in cells. </p> | <p>NF-κB is one of the most pathways inside the human body. This part contains five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. NF-κB is the down-stream of TNF signal, so in the experiments we add TNF to induce NF-κB production in cells. </p> | ||
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| + | ===Usage=== | ||
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<p>This part is used as a part improvement of BBa_K2976009.</p> | <p>This part is used as a part improvement of BBa_K2976009.</p> | ||
<p>Our new part we designed here improved the efficiency more than twice than the original part. We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter. </p> | <p>Our new part we designed here improved the efficiency more than twice than the original part. We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter. </p> | ||
Latest revision as of 12:37, 9 December 2020
JTi1
Promoter JTi2 consists of five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. The reconstructed promoter is more than twice as efficient as the original part BBa_K2976009.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Improved by NFLS 2020
Biology
NF-κB is one of the most pathways inside the human body. This part contains five repeats of 5′-GGGTTTCCCC-3′as NF-κB binding site and a minimal CMV promoter. The binding sites repeats is derived from gene vascular cell adhesion molecule 1, VCAM1. NF-κB is the down-stream of TNF signal, so in the experiments we add TNF to induce NF-κB production in cells.
Usage
This part is used as a part improvement of BBa_K2976009.
Our new part we designed here improved the efficiency more than twice than the original part. We added a Secreted Alkaline Phosphatase (SEAP) which commonly used as a reporter gene behind the promoter. The enzyme activity of SEAP reflects the efficacy of the promoter.
The origin part is: BBa_K2976009
The standard curve:
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]