Difference between revisions of "Part:BBa K3657037"

Latest revision as of 03:51, 28 October 2020

RNA Linker binding Pumby AUAGAU and Pumby AUGGUU
This part is an RNA containing binding sites for Pumby protein binding AUAGAU (BBa_K3657004) and Pumby protein binding AUGGUU (BBa_K3657005).

Usage and Biology

Pumby proteins are modular proteins. Each subunit binds a specific ribonucleotide. Therefore the protein can be designed to bind a specific RNA sequence.

It was planned to test the ability of this linker to bring proteins together by using Pumby proteins fused to respective Split-GFP subunits. The following parts were planned to be used: BBa_K3657034, BBa_K3657035.

Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: iGEM Heidelberg 2020

Figure 1: Secondary structure of the part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <p>Due to the COVID19 pandemics no experimental data about this part was obtained. More information about the planned experiment can be found here: <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR">iGEM Heidelberg 2020</a></p>
 </html>
+<html>
+<center>
+<img style="width:75%" src="https://static.igem.org/mediawiki/parts/d/de/T--Heidelberg--Parts-BBa_K3657037.jpg">
+</center>
+<p><b>Figure 1:</b> Secondary structure of the part.</p>
+</html>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3657037 SequenceAndFeatures</partinfo>