Difference between revisions of "Part:BBa K3425052"
 
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<b>QB recognition site</b>
 
<b>QB recognition site</b>
 
<br>
 
<br>
−
<b>Refereces</b>
+
<b>References</b>
 
<br>[1]  
 
<br>[1]  
 
<br>Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
 
<br>Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078

Latest revision as of 03:24, 28 October 2020


Qβ5' recognition sequence of Qβ replicase with RBS

BBa_K3425052 is a construct containing the Qβ5' recognition sequence of Qβ replicase, BBa_K3425038, fused with the RBS designed for Type IIS iGEM Standard assembly, BBa_K3425032.

QB recognition site
References
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 61
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 64
    Illegal XhoI site found at 68
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 61
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]