Difference between revisions of "Part:BBa K3606807"

 
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McbB works as synthase responsible for the maturation of MccB17.
 
McbB works as synthase responsible for the maturation of MccB17.
 
<h2>Design:</h2>
 
<h2>Design:</h2>
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbA product was produced.
+
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced.
  
 
<h2>Results:</h2>
 
<h2>Results:</h2>

Latest revision as of 02:42, 5 December 2020


mcbB

Usage and Biology:

McbB works as synthase responsible for the maturation of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced.

Results:

More information:https://parts.igem.org/Part:BBa_K3606814

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]