Difference between revisions of "Part:BBa K165068"
 
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Induced by galactose, this activator, tagged with mCherryx2, activates promoters with LexA binding sites.
 
Induced by galactose, this activator, tagged with mCherryx2, activates promoters with LexA binding sites.
  
−
In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional.  Under the confocal fluorescence microscope, we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:
+
In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional.  After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:
  
  

Latest revision as of 06:18, 30 October 2008

pGAL1 + LexA activator on pRS305

Induced by galactose, this activator, tagged with mCherryx2, activates promoters with LexA binding sites.

In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:


Fluorotest.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]