Difference between revisions of "Part:BBa K3417000"
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===Functional Parameters=== | ===Functional Parameters=== | ||
kcat = 2940 min^(-1) | kcat = 2940 min^(-1) | ||
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===References=== | ===References=== | ||
Latest revision as of 00:49, 28 October 2020
ArgK Coding Sequence
This part is the coding sequence of the protein Phaselotoxin Resistant Ornithine Carbamoyltransferase (ROTCase), which substitutes the metabolic role of E. coli ornithine carbamoyltransferase in the bacterial urea cycle.
This figure shows the Sequential Bi-Bi Mechanism of ROTCase, in which carbomylphosphate is the first substrate to bind, allowing ornithine to then bind to the complex.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 859
Functional Parameters
kcat = 2940 min^(-1)
References
Kelly, J., Rubin, A., Davis, J., Ajo-franklin, C., Cumbers, J., Czar, M., De Mora, K., Glieberman, A., Monie, D., &Endy, D. (2009). Measuring the activity of biobrick promoters using an in vivo reference standard.Journalof Biological Engineering,3(1), 4. https://doi.org/10.1186/1754-1611-3-4
Templeton, M. D., Reinhardt, L. A., Collyer, C. A., Mitchell, R. E., & Cleland, W. W. (2005). Kinetic analysisof the l-ornithine transcarbamoylase from pseudomonas savastanoi pv. phaseolicola that is resistant to thetransition state analogue (r)-n-(n‘-sulfodiaminophosphinyl)-l-ornithine [PMID: 15766270].Biochemistry,44(11), 4408–4415. https://doi.org/10.1021/bi047432x