Difference between revisions of "Part:BBa K3425061"
 
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BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
 
BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.
  
−
From observations have can it be stated that this construct tends to mutate in high copy number plasmids
+
From observations we had, it can be stated that this construct tends to mutate in high copy number plasmids.
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 +
 
 +
<br>
 +
<b>Refereces</b>
 +
<br><b>QB</b>
 +
<br>[1]
 +
<br>Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
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<br><i>Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.</i>
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<br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:18, 28 October 2020


Constitutive expression construct of Qβ replicase

BBa K3425061 is a Constitutive expression construct of Qβ replicase, and is constructed from the constitutive promoter, BBa_J23108, the RBS for Type IIS iGEM Standard Assembly, BBa_K3425033, β-subunit of Qβ replicase with cleavable degradation tag, BBa_K3425051, as well as the double terminator BBa_B0015.

From observations we had, it can be stated that this construct tends to mutate in high copy number plasmids.



Refereces
QB
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1377
    Illegal XhoI site found at 885
    Illegal XhoI site found at 1051
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]