Difference between revisions of "Part:BBa K3657021"

Latest revision as of 00:37, 28 October 2020

RNA Motif GUAGAG targeted PPR - Ribonuclease A Complex

This part is a fusion protein. It consists of a PPR complex that binds GUAGAG RNA loop and the Ribonuclease A.

Usage and Biology

Ribonuclease A (BBa_K3657019) is a unspecific highly active endonuclease.

Single PPR subunits bind to specific ribonucleotides. Concatenation of the subunits forms a protein that binds a specific RNA sequence.

In that way this construct can deliver Ribonuclease A to a specific RNA. It was planned to be used to bind to the following Broccoli modifications: BBa_K3657017, BBa_K3657018. More information can be found here: iGEM Heidelberg 2020.

Due to the COVID pandemic we were not able to experimentally examine performance of this part.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 15: / Line 15: @@
 </p>
 <p>
-In that way this construct can deliver Ribonuclease A to a specific RNA. It was planned to be used to bind to the following Broccoli modifications: <a href="https://parts.igem.org/Part:BBa_K3657017">BBa_K3657017</a>, <a href="https://parts.igem.org/Part:BBa_K3657018">BBa_K3657018</a>.
+In that way this construct can deliver Ribonuclease A to a specific RNA. It was planned to be used to bind to the following Broccoli modifications: <a href="https://parts.igem.org/Part:BBa_K3657017">BBa_K3657017</a>, <a href="https://parts.igem.org/Part:BBa_K3657018">BBa_K3657018</a>. More information can be found here: <a href="https://2020.igem.org/Team:Heidelberg/Pumilio_PPR">iGEM Heidelberg 2020.</a>
 </p>
+<p>Due to the COVID pandemic we were not able to experimentally examine performance of this part.</p>
 </html>
 <span class='h3bb'>Sequence and Features</span>