Difference between revisions of "Part:BBa K3475008"

Latest revision as of 01:05, 28 October 2020

Prm+ RBS GFP Ter

GFP activated by cI

Usage and Biology

FIGURE 6. The relative fluorescence of HrtR- (E.coli-pSB1K3-HrtO-cI-pA1c-R0051-mRFP-I12007-GFP), HrtR+ (E.coli-pHSB-cI-pA1c-R0051-mRFP-I12007-GFP) and WT (E.coli-wildtype).
Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm for GFP; and by excitation wavelength of 588 nm, emission wavelength of 635 nm for mRFP. Relative fluorescence is calculated by the formula below, LB medium as blank.As shown in the figure, when heme is sufficient (simulated by KO of HrtR), the expression of GFP (promoter: I12007) is activated and the expression of RFP (promoter: R0051) is repressed; when heme lacks, the expression of GFP (promoter: I12007) is repressed and the expression of RFP (promoter: R0051) is activated. This can to some extent prove the engineering success of the connection between heme biosensor system and bilateral ARG system.

FIGURE 7. Calculating formula.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 738

@@ Line 9: / Line 9: @@
 <div style="text-align:center">[[Image:T--Tongji_China--202_6.jpg | 750px]]</div>
 <p style="width:600px; margin-left:150px; margin-bottom:60px;
-text-align:justify "><em><strong>FIGURE 4.</strong> '''The relative fluorescence of HrtR- (E.coli-pSB1K3-HrtO-cI-pA1c-R0051-mRFP-I12007-GFP), HrtR+ (E.coli-pHSB-cI-pA1c-R0051-mRFP-I12007-GFP) and WT (E.coli-wildtype).'''<br/> Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm for GFP; and by excitation wavelength of 588 nm, emission wavelength of 635 nm for mRFP. Relative fluorescence is calculated by the formula below, LB medium as blank.As shown in the figure, when heme is sufficient (simulated by KO of HrtR), the expression of GFP (promoter: I12007) is activated and the expression of RFP (promoter: R0051) is repressed; when heme lacks, the expression of GFP (promoter: I12007) is repressed and the expression of RFP (promoter: R0051) is activated. This can to some extent prove the engineering success of the connection between heme biosensor system and bilateral ARG system.
+text-align:justify "><em><strong>FIGURE 6.</strong> '''The relative fluorescence of HrtR- (E.coli-pSB1K3-HrtO-cI-pA1c-R0051-mRFP-I12007-GFP), HrtR+ (E.coli-pHSB-cI-pA1c-R0051-mRFP-I12007-GFP) and WT (E.coli-wildtype).'''<br/> Measured by excitation wavelength of 475 nm, emission wavelength of 515 nm for GFP; and by excitation wavelength of 588 nm, emission wavelength of 635 nm for mRFP. Relative fluorescence is calculated by the formula below, LB medium as blank.As shown in the figure, when heme is sufficient (simulated by KO of HrtR), the expression of GFP (promoter: I12007) is activated and the expression of RFP (promoter: R0051) is repressed; when heme lacks, the expression of GFP (promoter: I12007) is repressed and the expression of RFP (promoter: R0051) is activated. This can to some extent prove the engineering success of the connection between heme biosensor system and bilateral ARG system.
   </em> </p>
 <div style="text-align:center">[[Image:T--Tongji_China--202_t.jpg | 750px]]</div>
 <p style="width:600px; margin-left:150px; margin-bottom:60px;
-text-align:justify "><em><strong>FIGURE 5.</strong> '''Calculating formula.'''<br/>
+text-align:justify "><em><strong>FIGURE 7.</strong> '''Calculating formula.'''<br/>
   </em> </p>