Difference between revisions of "Part:BBa K3657002"
 
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The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificallity is due to a variation in the amino acid in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, being able to bind a sequence of up to 18 RNA nucleotides.  
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The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificity is determined by the amino acids in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, which is able to bind a sequence of up to 18 ribonucleotides.  
 
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This part is the Uracil binding Pumby Domain.   
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This part is the uracil binding Pumby Domain.   
 
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Combined with the other Pumby Domains Adenine:<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a><p>
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It can be assembled to a Pumby Protein when combined with the other Pumby Domains: <p> Adenine:<a href="https://parts.igem.org/Part:BBa_K3657000">BBa_K3657000</a><p>
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Guanine:<a href="https://parts.igem.org/Part:BBa_K3657002">BBa_K3657002</a><p>
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Guanine:<a href="https://parts.igem.org/Part:BBa_K3657001">BBa_K3657001</a><p>
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Cytosine<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p> it can be assembled to a Pumby Protein, binding a specific RNA sequence (see the compotitive parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>)
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Cytosine:<a href="https://parts.igem.org/Part:BBa_K3657003">BBa_K3657003</a><p>
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See also examples of the composite parts: <a href="https://parts.igem.org/Part:BBa_K3657004">BBa_K3657004</a>, <a href="https://parts.igem.org/Part:BBa_K3657005">BBa_K3657005</a>)
 
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===Usage and Biology===
 
===Usage and Biology===
  
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Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila Melanogaster), which is a RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
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Pumby Domains are engeneered from the Pumilio Homology Domain (<i>Drosophila melanogaster</i>), which is an RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Latest revision as of 01:05, 28 October 2020


RNA binding Pumilio Homology Domain (Uracil)

The Pumby Domains are 36 amino acid long subunits, that specifically bind one of the RNA bases. The specificity is determined by the amino acids in position 16 and 20. Multiple Pumby Domains can be assembled into one Pumby protein, which is able to bind a sequence of up to 18 ribonucleotides.


This part is the uracil binding Pumby Domain.


It can be assembled to a Pumby Protein when combined with the other Pumby Domains:

Adenine:BBa_K3657000

Guanine:BBa_K3657001

Cytosine:BBa_K3657003


See also examples of the composite parts: BBa_K3657004, BBa_K3657005)

Usage and Biology

Pumby Domains are engeneered from the Pumilio Homology Domain (Drosophila melanogaster), which is an RNA binding protein, that is made out of 6 repeating subunits containing three alpha helices and two imperfect subunits at the N- and C-Terminus. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 70
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]