Difference between revisions of "Part:BBa K3425052"

Latest revision as of 03:24, 28 October 2020

Qβ5' recognition sequence of Qβ replicase with RBS

BBa_K3425052 is a construct containing the Qβ5' recognition sequence of Qβ replicase, BBa_K3425038, fused with the RBS designed for Type IIS iGEM Standard assembly, BBa_K3425032.

QB recognition site
References
[1]
Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 61
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 64
Illegal XhoI site found at 68
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 61
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 61
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 BBa_K3425052 is a construct containing the Qβ5' recognition sequence of Qβ replicase, BBa_K3425038, fused with the RBS designed for Type IIS iGEM Standard assembly, BBa_K3425032.
+<br><br>
+<b>QB recognition site</b>
+<br>
+<b>References</b>
+<br>[1]
+<br>Yao, Y., Zhang, W., Zhang, M., Jin, S., Guo, Y., Zu, Y., Ren, K., Wang, K., Chen, G., Lou, C., and Wu, Q. (2019) A Direct RNA-to-RNA Replication System for Enhanced Gene Expression in Bacteria. ACS Synth. Biol. 8, 1067–1078
+<br><i>Note, that the authors declared the following competing financial interest(s): Y. Y., W. Z., and Q. W. have filed a patent on RNA replication system and its usage.</i>
+<br>
 <!-- Add more about the biology of this part here