Difference between revisions of "Part:BBa K3331000"
(→Characterization) |
|||
| Line 23: | Line 23: | ||
<div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div> | <div>[[File:T--XJTU-China--Bggel.png |700px|thumb|center|<b>Figure 1:</b>PCR confirmation of the target fragment.]]</div> | ||
| − | <div>[[File:T--XJTU-China--Bgimprove02.jpeg]]</div> | + | <div>[[File:T--XJTU-China--Bgimprove02.jpeg |700px|thumb|center|]]</div> |
| − | <div>[[File:T--XJTU-China--Bgimprove01.jpeg]]</div> | + | <div>[[File:T--XJTU-China--Bgimprove01.jpeg |700px|thumb|center|]]</div> |
the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable. | the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable. | ||
Latest revision as of 23:48, 27 October 2020
B.S. galU
galU (ytdA) [Bacillus subtilis (strain 168)] Encodes UTP--glucose-1-phosphate uridylyltransferase that catalyzes the formation of UDP-glucose from glucose-1-phosphate and UTP in Bacillus subtilis.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterization
We cloned and confirmed the target fragment by PCR. The length of our fragment is 951bp. The following results showed the successful result.
the mRNA level of B.s GalU is ~2.4 fold higher than that of E.coli, indicating the significant improvement of enzyme expression and activity. However, due to the lower expression of another key enzyme PGM for EPS production in BE strain, the final EPS yield is lower than that of EE strain but still comparable.