Difference between revisions of "Part:BBa K3699003"
 
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PylRS: the pyrrolysyl-tRNA synthetase gene of <i>Methanosarcina mazei</i> (gene: MSMAC_3241, protein id: AKB73131.1), J23119 promoter
 
PylRS: the pyrrolysyl-tRNA synthetase gene of <i>Methanosarcina mazei</i> (gene: MSMAC_3241, protein id: AKB73131.1), J23119 promoter
  
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<h3 id="degradation0301">Design</h3>
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From virus to human, the same set of genetic code is employed, which contains the start codon, three stop codons (UAA, UAG, UGA), and the remaining 61 codons encoding 20 kinds of amino acids. However, some exceptions have been found, such as pyrrolysyl tRNA synthetase/tRNA (pylrs/tRNA) from <i>Methanosarcina mazei</i>, which can recognize the amber codon UAG and translate it into pyrrolysine. Moreover, in E. coli, yeasts, and mammalian cells, this PylRS/tRNA pair is orthogonal, which means it does not interfere with the translation system of the chassis [1]. The orthogonal translation system has been used in vaccine safety design [2]. Now, we will use it to achieve the anti-escape design of cyanophages.
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<h3 id="degradation0301">References</h3>
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<p>[1] Mukai T , Kobayashi T , Hino N , et al. Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases[J]. Biochemical & Biophysical Research Communications, 2008, 371(4):818-822.
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<p>[2] Si L , Xu H , Zhou X , et al. Generation of influenza A viruses as live but replication-incompetent virus vaccines.[J]. Science, 2016.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 23:10, 27 October 2020


PylRS

PylRS: the pyrrolysyl-tRNA synthetase gene of Methanosarcina mazei (gene: MSMAC_3241, protein id: AKB73131.1), J23119 promoter

Design

From virus to human, the same set of genetic code is employed, which contains the start codon, three stop codons (UAA, UAG, UGA), and the remaining 61 codons encoding 20 kinds of amino acids. However, some exceptions have been found, such as pyrrolysyl tRNA synthetase/tRNA (pylrs/tRNA) from Methanosarcina mazei, which can recognize the amber codon UAG and translate it into pyrrolysine. Moreover, in E. coli, yeasts, and mammalian cells, this PylRS/tRNA pair is orthogonal, which means it does not interfere with the translation system of the chassis [1]. The orthogonal translation system has been used in vaccine safety design [2]. Now, we will use it to achieve the anti-escape design of cyanophages.

References

[1] Mukai T , Kobayashi T , Hino N , et al. Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases[J]. Biochemical & Biophysical Research Communications, 2008, 371(4):818-822.

[2] Si L , Xu H , Zhou X , et al. Generation of influenza A viruses as live but replication-incompetent virus vaccines.[J]. Science, 2016.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 674
    Illegal NgoMIV site found at 1258
  • 1000
    COMPATIBLE WITH RFC[1000]