Difference between revisions of "Part:BBa K165078:Experience"

m
 
Line 4: Line 4:
  
 
===Applications of BBa_K165078===
 
===Applications of BBa_K165078===
−
 
+
In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional.  After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:
−
Generates YFP when activated by the LexA synthetic transcriptional activator in yeast.
+
−
 
+
−
We saw this work and took this picture on a confocal microscope:
+
  
  

Latest revision as of 06:16, 30 October 2008

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K165078

In a strain into which we transformed the LexA activator and LexA activator reporter, we found this part to be functional. After growing in 2% galactose/2%raffinose, under the confocal fluorescence microscope we saw mCherry expression in the nucleus (on the activating transcription factor), and its effect of inducing YFP expression in the cytosol from the activator reporter. See below:


Fluorotest.png

The mCherry on the activator is red in the nucleus, and the YFP is green in cytosol.

User Reviews

UNIQ5885af37e022f95c-partinfo-00000000-QINU UNIQ5885af37e022f95c-partinfo-00000001-QINU