Difference between revisions of "Part:BBa K3396018"
 
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<partinfo>BBa_K3396018 short</partinfo>
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<partinfo>BBa K3396018 short</partinfo>
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CMV->Kozak->GFPnano->FKBP->FKBP->P2A ->Truncated TRIM21->FRB->BGH-pA
  
 
This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP.
 
This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP.
 
And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.
 
And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.
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This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP.
 
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And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.
 
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===Usage and Biology===
 
===Usage and Biology===
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Our composite part BBa_K3396018 demonstrates how the Predator Pro system works. It regulates the target protein (EGFP) degradation under the control of external rapamycin signal.  
 
Our composite part BBa_K3396018 demonstrates how the Predator Pro system works. It regulates the target protein (EGFP) degradation under the control of external rapamycin signal.  
  
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===Sequence and Features===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3396018 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3396007 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3396018 parameters</partinfo>
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<partinfo>BBa_K3396007 parameters</partinfo>
 
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Latest revision as of 21:20, 27 October 2020


No part name specified with partinfo tag. CMV->Kozak->GFPnano->FKBP->FKBP->P2A ->Truncated TRIM21->FRB->BGH-pA

This part is designed to demonstrate a small molecule induced target protein degradation with the Predator Pro system. Herein, FRB-FKBP-FKBP pairs were used to mediate the rapamycin induced trimerization of truncated Trim21, GFP nanobody and target protein EGFP. And double copy of FKBP could increase the protein interaction intensity of FKBP and FRB.

Usage and Biology

Our composite part BBa_K3396018 demonstrates how the Predator Pro system works. It regulates the target protein (EGFP) degradation under the control of external rapamycin signal.

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 204
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 161
  • 1000
    COMPATIBLE WITH RFC[1000]