Difference between revisions of "Part:BBa K3484003"

Latest revision as of 20:29, 27 October 2020

Intein-mediated T3(THRB) → eGFP (transcriptional unit)

This part is the transcriptional unit for BBa_K3484002. This part expresses constitutively an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (BBa_K3484000). Visit its page to see the characterization and final design.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 235
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 725
Illegal NgoMIV site found at 2228
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1640

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3484003 short</partinfo>
-This part is the transcriptional unit for <partinfo>BBa_K3484002</partinfo>. This part expresses constitutively an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (<partinfo>BBa_K3484001</partinfo>). Visit its page to see the characterization and final design.
+This part is the transcriptional unit for <partinfo>BBa_K3484002</partinfo>. This part expresses constitutively an intein-mediated T3 biosensor with enhanced GFP (eGFP) as a reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (<partinfo>BBa_K3484000</partinfo>). Visit its page to see the characterization and final design.
 <!-- Add more about the biology of this part here