Difference between revisions of "Part:BBa K3606810"

Latest revision as of 02:38, 5 December 2020

mcbE

Usage and Biology:

McbE works as channel protein responsible for efflux of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbE into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbE expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbE product was produced.

Results:

We have successfully expressed mcbE for the fundamental validation of the antimicrobial peptide(mccb17) expression.

Figure1. gel map for mcbA, mcbC, mcbE and mcbF

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 __NOTOC__
 <partinfo>BBa_K3606810 short</partinfo>
+<h2>Usage and Biology:</h2>
 McbE works as channel protein responsible for efflux of MccB17.
+<h2>Design:</h2>
+Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbE into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbE expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbE product was produced.
+<h2>Results:</h2>
+We have successfully expressed mcbE for the fundamental validation of the antimicrobial peptide(mccb17) expression.
+https://2020.igem.org/wiki/images/f/f3/T--Fudan--Poster_Mcb-gels.jpg
+Figure1. gel map for mcbA, mcbC, mcbE and mcbF