Difference between revisions of "Part:BBa K3606808"
 
(2 intermediate revisions by 2 users not shown)
Line 4: Line 4:
  
 
McbC works as synthase responsible for the maturation of MccB17.
 
McbC works as synthase responsible for the maturation of MccB17.
 +
 +
<h2>Design:</h2>
 +
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbG into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbC expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbC product was produced.
 +
 +
<h2>Results:</h2>
 +
https://parts.igem.org/Part:BBa_K3606814
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:02, 28 October 2020


mcbC

McbC works as synthase responsible for the maturation of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbG into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbC expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbC product was produced.

Results:

https://parts.igem.org/Part:BBa_K3606814

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]