Difference between revisions of "Part:BBa K3505017"
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     [[Image:T--Thessaly--GB-GCTT-CGCT.jpeg |jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the Golden Braid Grammar.</i>]]
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     [[Image:T--Thessaly--GB-GCTT-CGCT.jpeg |jpeg|900px|thumb|none|<i><b>Figure 1.</b>The overhangs of this part in the GoldenBraid Grammar.</i>]]
  
 
  [[Image:T--Thessaly-double terminator.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- double terminator. (illustration from SnapGene)</i>]]
 
  [[Image:T--Thessaly-double terminator.png|900px|thumb|none|<I><b>Figure 2.</b> The level 0 module : pupd2- double terminator. (illustration from SnapGene)</i>]]
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===Source===
 
===Source===
 
Synthesized by IDT.
 
Synthesized by IDT.
 +
 +
===Sequence and Features===
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<partinfo>BBa_K3505017 SequenceAndFeatures</partinfo>

Latest revision as of 20:31, 27 October 2020


Terminator.png

Double Terminator GB compatible with B6-C1

biologyrrnBT1-T7TE
chassisE.coli
directionForward
forward_efficiency0.984[CC]
0.97[JK]
reversed_version2146
reverse_efficiency0.295[CC]
0.62[JK]

Design Notes

The coding sequence was domesticated . We removed BsmBI ,BsaI , BtgZI, BpiI sites in order to be compatible with GoldenBraid and MoClo. The sequence is presents in pUPD2 and has overhangs compatible for Golden Braid cloning. The CDS has position B6-C1.

Figure 1.The overhangs of this part in the GoldenBraid Grammar.
Figure 2. The level 0 module : pupd2- double terminator. (illustration from SnapGene)

Verification of Cloning

Fig.3:(U=Uncut C=Cut) Restriction digestion of Terminator (C3 )with : EcoRI & PstI ( C3 ), Expected bands : 2029 + 209 bp , Positive result : C3

Source

Synthesized by IDT.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]